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Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: full-length reverse-transcribed recombinant cDNA generates infectious virus in mammalian cells.

机译：心脏型柯萨奇B3病毒基因组的分子克隆：全长反转录重组cDNA在哺乳动物细胞中产生传染性病毒。

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The molecular cloning of double-stranded cDNA synthesized from the single-stranded RNA genome of the cardiotropic Coxsackie B3 virus (Nancy strain) is reported. Full-length reverse-transcribed cloned viral cDNA of approximately equal to 7500 nucleotides generated infectious antigenically identical Coxsackie B3 virus upon transfection of recombinant plasmid DNA into mammalian cells, demonstrating the molecular cloning of a biologically active viral cDNA copy. Furthermore, the cloned cDNA is characterized by restriction enzyme analysis and partial nucleotide sequencing of the 5' end. The Coxsackie B3 virus cDNA described can now be used to study the molecular basis of human enteroviral heart disease, and it provides a valuable diagnostic means for patients with suspected viral heart disease.

机译：报道了从心型柯萨奇B3病毒（南希菌株）的单链RNA基因组合成的双链cDNA的分子克隆。重组质粒DNA转染哺乳动物细胞后，全长约7500个核苷酸的全长反转录克隆的病毒cDNA产生了传染性抗原相同的柯萨奇B3病毒，这证明了具有生物学活性的病毒cDNA拷贝的分子克隆。此外，通过限制酶分析和5'末端的部分核苷酸测序来表征克隆的cDNA。所描述的柯萨奇B3病毒cDNA现在可用于研究人类肠道病毒性心脏病的分子基础，它为疑似病毒性心脏病的患者提供了宝贵的诊断手段。

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作者
Kandolf, R; Hofschneider, P H;
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年度 1985
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正文语种 en
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Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: full-length reverse-transcribed recombinant cDNA generates infectious virus in mammalian cells.

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